Peptide Reconstitution Calculator

Work out the concentration of a reconstituted vial and exactly how many units to draw on a U-100 insulin syringe. Enter the amount in the vial, the bacteriostatic (BAC) water you add, and your amount per dose.

mL

Optional. Enter your own amount to see its volume.

Enter the amount in the vial and the solution added to see the concentration.

This is arithmetic on your inputs. VialVine doesn’t suggest amounts or preparation methods, and it isn’t a medical device. Double-check every calculation and talk to a healthcare professional about medical decisions.

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How the reconstitution math works

  • Concentration (mg/mL) = amount in the vial (mg) ÷ solution added (mL).
  • Volume to draw (mL) = amount per dose (mg) ÷ concentration.
  • Units on a U-100 syringe = volume (mL) × 100, because a U-100 syringe holds 100 units per mL.
  • Example: 10 mg with 2 mL of BAC water is 5 mg/mL. A 0.25 mg dose is 0.05 mL, or 5 units. That vial holds 40 doses of 0.25 mg.

BAC water and units: common vial sizes

What different amounts of bacteriostatic water give you for common vial sizes. The calculator above works for any numbers.

VialWater addedConcentrationmcg per unitUnits for 0.25 mg
5 mg1 mL5 mg/mL50 mcg5
5 mg2 mL2.5 mg/mL25 mcg10
5 mg2.5 mL2 mg/mL20 mcg12.5
10 mg1 mL10 mg/mL100 mcg2.5
10 mg2 mL5 mg/mL50 mcg5
10 mg3 mL3.333 mg/mL33.3 mcg7.5
15 mg3 mL5 mg/mL50 mcg5
20 mg2 mL10 mg/mL100 mcg2.5

Units are on a U-100 insulin syringe (100 units = 1 mL).

Frequently asked questions

How many units is 0.25 mg on an insulin syringe?

It depends on the concentration. At 5 mg/mL (10 mg in 2 mL), 0.25 mg is 0.05 mL, which is 5 units on a U-100 syringe. Enter your own numbers above to see yours.

What is a U-100 syringe?

A U-100 insulin syringe is marked in units where 100 units equal 1 mL. Common sizes are 0.3 mL (30 units), 0.5 mL (50 units) and 1 mL (100 units).

How much bacteriostatic water should I add?

That's your choice; the calculator doesn't recommend an amount. More liquid lowers the concentration, which makes each draw larger and easier to measure. Less liquid makes each draw smaller.

Does it work in mcg?

Yes. Switch the vial or dose unit to mcg. 1 mg = 1,000 mcg.

How do I calculate a peptide dose in units?

Find the concentration (mg in the vial ÷ mL of water added), divide your amount by it to get mL, then multiply by 100 for units on a U-100 syringe. 0.5 mg from a 10 mg vial with 2 mL of water: 10 ÷ 2 = 5 mg/mL; 0.5 ÷ 5 = 0.1 mL; 0.1 × 100 = 10 units.

How many units is 250 mcg?

250 mcg is 0.25 mg. At 5 mg/mL (10 mg in 2 mL) that's 5 units; at 2.5 mg/mL (5 mg in 2 mL) it's 10 units; at 10 mg/mL (10 mg in 1 mL) it's 2.5 units.

What is a peptide calculator?

A peptide calculator works out the concentration of a reconstituted vial and how much liquid holds a given amount, shown in mL and in units on an insulin syringe. It's the same arithmetic you'd do by hand, without the room for slips.

Can I convert mg to units without knowing the concentration?

No. Units measure volume, and mg measure weight, so the conversion depends on how much is dissolved in each mL. To convert units to mL only, use the mg to units converter: on a U-100 syringe, 100 units = 1 mL.

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